1. In vitro?Expression of H22-Luc-hGPC3 cell line

hGPC3 expression was measured in H22-Luc-hGPC3 cell?by?FACS?. FACS results showed that significant hGPC3?expression on the H22-Luc-hGPC3?cell?line.

Figure?1 hGPC3 expression in H22-Luc-hGPC3 cell line

2. Characterization of in vivo?growth kinetics

To verify tumor formation of?H22-Luc-hGPC3?cell line,?cells?were subcutaneously inoculated into?female?balb/c?mice.

Figure?2. Tumor growth curve of H22-Luc-hGPC3 allogeneic model (n=5)

Figure 3. Body weight curve of H22-Luc-hGPC3 allogeneic model (n=5)

3. Expression of hGPC3 in tumors

To verify the hGPC3 protein expression, the tumors were dissected and separated for single cell suspension when he tumor volume is 500-1000mm³. Robust hGPC3 protein expression can be detected in the humanized modified tumors.

Figure?4. The expression of hGPC3 in humanized modified tumors by FACS