MC38-hCD47/hCLDN18.2/hHER3
品系基本信息
驗(yàn)證數(shù)據(jù)
Ⅰ.In vitro?Expression of MC38-hCD47/hCLAUDIN18.2/hHER3 cell line
The human CD47 , human claudin18.2 and human HER3?protein expression in the MC38-hCD47/hCLAUDIN18.2/hHER3 cells were measured by Flow Cytometry.
Figure?1. The expression of hCD24 , hCLAUDIN18.2 and hHER3 in MC38-hCD47/hCLAUDIN18.2/hHER3 cell line
Ⅱ.Characterization of in vivo?growth kinetics
To test the take rates, and?the tumor growth characteristics of MC38-hCD47/hCLAUDIN18.2/hHER3?cell line, cells?were subcutaneously inoculated into the C57BL/6 mice.
Figure?2. Tumor growth curve of MC38-hCD47/hCLAUDIN18.2/hHER3 syngeneic model
Figure?3. Body weight change of MC38-hCD47/hCLAUDIN18.2/hHER3 syngeneic model
Ⅲ.Expression of hCD47/hCLAUDIN18.2/hHER3 protein in tumors
To verify the human CD47 , human claudin18.2 and human HER3?protein expression, the tumors were dissected and separated for single cell suspension.
Figure?4. The expression of hCD47 , hCLAUDIN18.2 and hHER3 in humanized modified tumors by FACS
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