MC38-hPSMA (2)
品系基本信息
驗(yàn)證數(shù)據(jù)
Ⅰ.In vitro?Expression of MC38-hPSMA cell line
The hPSMA?protein expression in the wild-type MC38 and MC38- hPSMA?cells were measured by Flow Cytometry.
Figure?1. hPSMA expression in wild-type MC38 and MC38-hPSMA cell line
Ⅱ.?Characterization of in vivo?growth kinetics
To test the take rates, and?the tumor growth characteristics of MC38-hPSMA?cell line, cells?were subcutaneously inoculated into the C57BL/6 mice.
Figure?2. Tumor growth curve of MC38-hPSMA syngeneic model
Figure?3. Body weight change of MC38-hPSMA syngeneic model
Ⅲ.?Expression of hPSMA protein in tumors
To verify the hPSMA protein expression, the tumors were dissected and separated for single cell suspension.
Figure?4. The expression of hPSMA in humanized modified tumors by FACS
Ⅳ.Efficacy verification
Murine colon cancer MC38-hPSMA?cells were subcutaneously implanted into hCD28/hPSMA-C57BL/6 mice. Mice were grouped when tumor volume reached approximately 80-90 mm3, at which time they were treated with CD28×PSMA+mPD-1?antibody?indicated in panel.
Figure 5. Antitumor activity of CD28×PSMA+mPD-1 antibody. MC38-hPSMA cells provided a powerful preclinical model for in vivo evaluation
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